PROCEED AMP3 Bedienungsanleitung PDF herunterladen

Infinium

HD Assay Gemini, Automated Protocol

Experienced User Card

Catalog # WG-901-3002 Page 1 of 30

Part # 11311023 Rev. A

Day 1

Day 2 Day 3

Make AMP3

Robot:

20 min/16 samples

Incubation: 20–24 hours

Reagents

0.1N NaOH

MP1

AMM

Output

AMP3 Plate with

Amplified DNA

Make Multi AMP3

Robot:

55 min/48 samples

Incubation: 20–24 hours

Reagents

0.1N NaOH

MP1

AMM

Output

1–4 AMP3 Plates with

Amplified DNA

XStain HD

BeadChip

Robot:

150 min/8 BeadChips

Dry: 1 hour

Reagents

RA1

95% Formamide /

1mMEDTA

PB1

XC1

XC2

XC3

XC4

TEM

STM

ATM

Output

BeadChip

Precip AMP3

Robot: 15 min/

16 samples

Incubation:55min

Dry: 60 min

Reagents

2-propanol

PA1

Output

AMP3 Plate

Fragment AMP3

Robot: 10 min/

16 samples

Incubation:60min

Reagents

FRG

Output

AMP3 Plate

Resuspend AMP3

Robot: 15 min/plate

Incubation:1hour

Reagents

RA1

Output

AMP3 Plate

Hyb Duo BeadChip

Robot: 10 min/

8 BeadChips

Incubation: 16–24 hours

Reagents

PB2

Output

BeadChip

Wash BeadChip

Robot: 20 min/

8 BeadChips

Reagents

PB1

Output

BeadChip

Pre-Amp

Optional

InfiniumLIMS

Post-Amp

Cold Storage

Option

Overnight

Incubation

Fill in the lab tracking

form and the sample

sheet as you perform

the assay

Quantitate DNA

Hands-on: ~20 min/plate

Robot: 20 min/plate

Reagents

Lambda DNA

PicoGreen dsDNA

1X TE

Output

Sample QDNA Plate with

Quantitated DNA

Image BeadChip

iScan System Scan Time:

35 min/BeadChip

BeadArray Reader Scan

Time: 45 min/BeadChip

Output

mage and Data Files

1 2 3 4 5 6 ... 29 30

Experienced User Card 1

Inhaltsverzeichnis

Seite 1 - Experienced User Card

Infinium® HD Assay Gemini, Automated ProtocolExperienced User CardCatalog # WG-901-3002 Page 1 of 30Part # 11311023 Rev. ADay 1Day 2 Day 3Make AMP3Rob

Seite 2

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 10 of 30Part # 11311023 Rev. A[ ] 9. Vortex

Seite 3

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 11 of 30Part # 11311023 Rev. AEstimated TimeRo

Seite 4

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 12 of 30Part # 11311023 Rev. A[ ] 6. Place a

Seite 5

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 13 of 30Part # 11311023 Rev. AEstimated TimeRo

Seite 6

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 14 of 30Part # 11311023 Rev. A• Proceed to Hyb

Seite 7

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 15 of 30Part # 11311023 Rev. AEstimated TimeRo

Seite 8

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 16 of 30Part # 11311023 Rev. APrepare Hyb Cham

Seite 9

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 17 of 30Part # 11311023 Rev. A[ ] 7. Careful

Seite 10

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 18 of 30Part # 11311023 Rev. A[ ] 4. Shake a

Seite 11

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 19 of 30Part # 11311023 Rev. AEstimated TimeHa

Seite 12

Seite 13

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 20 of 30Part # 11311023 Rev. A[ ] a. Wearing

Seite 14

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 21 of 30Part # 11311023 Rev. A[ ] 7. Place a

Seite 15

Seite 16

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 23 of 30Part # 11311023 Rev. AEstimated TimeRo

Seite 17

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 24 of 30Part # 11311023 Rev. APreparation[ ]

Seite 18

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 25 of 30Part # 11311023 Rev. A[ ] 5. Place a

Seite 19

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 26 of 30Part # 11311023 Rev. A[ ] 3. For eac

Seite 20

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 27 of 30Part # 11311023 Rev. AEstimated TimeSc

Seite 21

Seite 22

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 29 of 30Part # 11311023 Rev. AEstimated Time1–

Seite 23

Infinium® HD Assay Gemini, Automated Protocol (Pre-Amp)Experienced User CardCatalog # WG-901-3002 Page 3 of 30Part # 11311023 Rev. AEstimated TimeHand

Seite 24

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 30 of 30Part # 11311023 Rev. AAs the BeadArray

Seite 25

Infinium® HD Assay Gemini, Automated Protocol (Pre-Amp)Experienced User CardCatalog # WG-901-3002 Page 4 of 30Part # 11311023 Rev. A[ ] 4. Transfer

Seite 26

Infinium® HD Assay Gemini, Automated Protocol (Pre-Amp)Experienced User CardCatalog # WG-901-3002 Page 5 of 30Part # 11311023 Rev. ARead QNT Plate[

Seite 27

Seite 28

Infinium® HD Assay Gemini, Automated Protocol (Pre-Amp)Experienced User CardCatalog # WG-901-3002 Page 7 of 30Part # 11311023 Rev. AEstimated TimeRobo

Seite 29

Infinium® HD Assay Gemini, Automated Protocol (Pre-Amp)Experienced User CardCatalog # WG-901-3002 Page 8 of 30Part # 11311023 Rev. A[ ] 4. (Non-Infi

Seite 30

Infinium® HD Assay Gemini, Automated Protocol (Post-Amp)Experienced User CardCatalog # WG-901-3002 Page 9 of 30Part # 11311023 Rev. AEstimated TimeRob